Abstract: In the process of construction and operation of underground water-sealed oil storage cavern, the water inflow of the caverns is an important factor affecting the safety and operation cost of the cavern. At present, it is difficult to reduce the permeability of surrounding rock to the design requirements by using the traditional grouting method of underground water-sealed oil storage cavern, so the water inflow of the caverns cannot be well controlled. Microbial-induced calcium carbonate precipitation(MICP)technology has the advantages of low grouting viscosity, strong environmental adaptability, and stable formation of mineralized substances, which has attracted extensive interest and research in academia and engineering. This paper first analyses the problems existing in water inflow control of underground water-sealed oil storage cavern. Then, the mechanism, influencing factors and the effect of MICP technology on fracture seepage control are expounded. Finally, the key issues of the application of MICP in underground water-sealed oil storage are discussed. The analysis shows that MICP, as an assisted grouting method for seepage control of underground water-sealed oil storage, can accurately control the change of surrounding rock permeability and achieve the purpose of effective control the water inflow.