不同胶结液驱动解磷微生物固化/稳定化重金属污染土力学特性研究

    STUDY ON THE MECHANICAL PROPERTIES OF HEAVY METAL CONTAMINATED SOIL SOLIDIFIED/STABILIZED BY PHOSPHATE-SOLUBILIZING MICROORGANISMS DRIVEN BY DIFFERENT CEMENTING FLUIDS

    • 摘要: 土体被重金属铅(Pb)污染后,强度降低、承载力下降,会引发工程及环境问题。本研究探讨了微生物诱导磷酸盐沉淀技术(MIPP)在Pb2+污染土体修复中的应用,利用MIPP技术提升污染土体的力学性能并稳定Pb2+,从而降低环境风险问题。为此,研究使用了解磷微生物枯草芽孢杆菌,并结合不同胶结液(SGP、S-Mg、S-Ca)进行室内对照实验,通过无侧限抗压强度、直接剪切和毒性浸出试验,评估不同条件下MIPP技术对Pb2+污染土力学性能和重金属稳定化的影响。结果表明,MIPP技术能够显著提升Pb2+污染土的无侧限抗压强度(UCS)和抗剪强度,同时有效降低了Pb2+浸出浓度,确保其符合环境安全标准。在不同胶结液条件下,SGP+CaCl2(S+Ca)组合的效果最佳,尤其在14 d养护期内,土体的力学性能和Pb2+固定效果达到最优,并满足工程应用需求。本研究为MIPP技术在Pb2+污染土的工程化修复提供了理论依据,也为重金属污染土的修复提供了新的思路和方法,具有较大的应用潜力。

       

      Abstract: Heavy metal contamination in soils, particularly Pb2+ pollution, presents significant environmental and geotechnical concerns. This study evaluates the effectiveness of microorganism-induced phosphate precipitation(MIPP)for stabilizing lead-contaminated soils and compares the performance of different cementing solutions in enhancing mechanical properties and immobilizing lead. Sodium glycerophosphate(SGP)was used as the phosphorus source, with MgCl2(S-Mg group)and CaCl2(S-Ca group)introduced as auxiliary cementing agents. A series of laboratory tests—including unconfined compressive strength(UCS), direct shear tests, and toxicity characteristic leaching procedure(TCLP)analyses—were conducted under varying curing times(3, 7, 14, and 28 days)and Pb2+ concentrations. The underlying mechanisms were further examined based on microbial activity, precipitation behavior, and mineral phase formation. The results indicate that MIPP treatment significantly improved the UCS and shear strength of Pb-contaminated soils, with the S-Ca group showing the most notable enhancement. After 14 days of curing, the peak UCS of the S-Ca group increased by up to 19.88% compared to other groups, while its peak shear strength rose by 36.36%. TCLP analyses confirmed that Pb2+ concentrations in leachates from S-Ca-treated soils remained below 0.1 mg ·L-1, complying with regulatory standards. Mechanistic investigations revealed that Ca2+ addition alleviated the toxic effects of Pb2+ on bacterial activity and promoted the formation of stable phosphate minerals, such as Ca2Pb3(PO4)3Cl, thereby improving both soil structure and long-term stability. MIPP proves to be an effective and sustainable technique for stabilizing Pb-contaminated soils, enhancing mechanical strength while effectively controlling Pb2+ leaching. Among the tested cementing solutions, the CaCl2-based system(S-Ca group)achieved an optimal balance between performance and cost-effectiveness, demonstrating strong potential for practical application in heavy metal-contaminated soil remediation.

       

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